质粒名称: | CaV2.1 (pcDNA6) |
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出品公司: | Addgene |
目录编号: | 26578 |
存储实验室: | Diane Lipscombe |
载体骨架基本信息 |
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载体名称: | pcDNA6/V5-HisA |
载体出品公司: | Invitrogen |
空载体大小: | 5100 bp |
完整质粒大小: | 12495 bp |
载体修饰: | -- |
载体类型: | 哺乳动物表达 |
筛选标记: | Blasticidin |
载体抗性: | 氨苄青霉素,100μg/mL |
生长温度: | 37 ℃ |
宿主菌: | Top10F' |
拷贝数: | -- |
插入基因信息 |
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插入基因名称: | Cacna1a |
物种来源: | M.musculus(小鼠) |
插入大小: | 7100 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | V5(主干上的C终端)His6(主干上的C终端) |
克隆方法: | 限制性内切酶 |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | T7 |
3’测序引物 | BGH-rev |
文献引用方法 |
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材料和方法部分: | CaV2.1 (pcDNA6) was a gift from Diane Lipscombe (Addgene plasmid # 26578 ; http://n2t.net/addgene:26578 ; RRID:Addgene_26578) |
参考文献部分: | Novel CaV2.1 clone replicates many properties of Purkinje cell CaV2.1 current. Richards KS, Swensen AM, Lipscombe D, Bommert K. Eur J Neurosci. 2007 Nov . 26(10):2950-61. 10.1111/j.1460-9568.2007.05912.x PubMed 18001290 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS CaV2.1 (pcDNA6) 12495 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS CaV2.1 (pcDNA6) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12495) AUTHORS Richards KS, Swensen AM, Lipscombe D, Bommert K TITLE Novel CaV2.1 clone replicates many properties of Purkinje cell CaV2.1 current. JOURNAL Eur J Neurosci. 2007 Nov . 26(10):2950-61. PUBMED 18001290 REFERENCE 2 (bases 1 to 12495) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..12495 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 7238..7267 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 7241..7270 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 8126..8167 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 8177..8194 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(8217..8234) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8223..8447 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8493..8921 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8580..8599) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8790..8811 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8930..8950) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8935..9264 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9115..9250 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9177..9196 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9312..9359 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9378..9776 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 9934..10055 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9971..9990) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10025..10044 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10104..10120) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10104..10120) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10117..10139) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10128..10144 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10152..10182) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10197..10218 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10335..10352) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10506..11094) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10586..10605) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11265..12125) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11888..11907 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12126..12230) /gene="bla" /label=AmpR promoter primer_bind complement(12305..12324) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg 661 gctagcgttt aaacttaagc ttggtaccga gctcggatcg atccactagt ccagtgtggt 721 ggaattcggc ttgaggccct ttgctctttg cagaatggcc cgctttggag acgagatgcc 781 gggccgctac ggcgcaggcg gaggaggctc agggccggcc gccggggtgg tcgtgggcgc 841 cgcgggcggc cgaggagccg ggggcagccg gcagggcggg cagcccggag cgcagaggat 901 gtacaagcag tcgatggcgc agagagcgcg gaccatggcc ctctacaacc ccatccctgt 961 ccgccagaac tgcctcacgg tcaaccgctc cctgttcctc ttcagtgaag acaacgtggt 1021 gagaaaatac gccaaaaaga tcacggaatg gcctcccttc gagtacatga tcctggccac 1081 catcattgct aactgcatcg tcctggccct ggagcagcac ctccctgatg atgacaagac 1141 acccatgtct gagcggctgg atgacacgga accatacttc attggaatct tctgttttga 1201 ggctggaatt aagatcgtgg ctcttggctt tgccttccac aaaggctcct acctgaggaa 1261 tggctggaac gtcatggact ttgtcgtggt gctaacaggc atcttggcca ctgtcgggac 1321 ggagtttgac ctacggacac tgagggcggt tcgtgtgctg cggccactca agctggtgtc 1381 tggaatccca agtttacaag tcgtcctgaa gtcaatcatg aaggcgatga tccctctgct 1441 gcagatcggc ctcctcctgt tttttgcaat ccttattttt gcaatcatag ggttagaatt 1501 ttatatgggg aaatttcata ccacctgctt tgaagagggg acagacgaca tccagggtga 1561 gtcgccagct ccgtgtggga cagaggagcc tgcccgcacc tgccccaacg ggaccaaatg 1621 tcagccgtac tgggaagggc ccaacaacgg catcactcag ttcgacaaca tcctgtttgc 1681 tgtgctcact gttttccagt gcatcaccat ggaaggctgg actgatctcc tctacaatag 1741 caacgatgcc tcagggaaca cttggaactg gttgtacttc atccccctca tcatcatcgg 1801 ctcctttttt atgctgaacc ttgttctggg tgtgctgtct ggggagtttg ccaaagaaag 1861 ggaacgtgtg gagaaccgga gagcttttct gaagctcaga agacaacagc agattgaacg 1921 tgagctcaat ggatacatgg agtggatctc aaaagcagaa gaggtgattc ttgcggagga 1981 cgagacagac gtggagcaga ggcacccttt tgatggagct cttcggagag ctactctgaa 2041 gaaaagcaag acagacctgc tcaaccctga ggaggccgag gaccagctgg ctgacatcgc 2101 gtctgtgggg tctcccttcg ccagagccag catcaaaagt gccaagctgg agaactcgac 2161 tttttttcac aagaaggaga gaagaatgcg tttctacatc cgccgcatgg tcaaaactca 2221 ggccttctac tggaccgtgc tcagtctggt agccctcaac acgctgtgcg tcgccattgt 2281 ccactacaac cagcccgagt ggctctccga cttcctctac tatgcagaat tcattttctt 2341 aggactcttt atgtccgaaa tgtttataaa aatgtacggg ctcgggacgc ggccttactt 2401 ccactcttcc ttcaactgct ttgactgtgg ggtcatcatt gggagcatct ttgaagtcat 2461 ctgggctgtc atcaaacccg gtacatcctt tggaatcagt gtgttacgag ctctcaggtt 2521 attgcgaatt ttcaaagtca caaagtactg ggcatctctc agaaacctgg ttgtctccct 2581 cctcaactcc atgaagtcca tcataagtct gttgttcctc cttttcctgt tcatcgttgt 2641 ctttgccctc ctggggatgc agctgttcgg tggccaattt aattttgatg aggggactcc 2701 gcccaccaac ttcgacactt ttccagcagc aataatgact gtgtttcaga tcctgactgg 2761 cgaggattgg aatgaggtca tgtatgatgg gatcaagtct caggggggcg tgcagggcgg 2821 catggtgttc tccatctact tcatcgtcct caccctcttc gggaactaca ccctgctgaa 2881 cgtgttcttg gccatcgcag tggacaacct ggccaatgcc caggagctca ccaaggatga 2941 gcaagaagag gaagaggcag ccaatcagaa gctggctcta cagaaagcca aggaggtagc 3001 agaagtgagt cccctgtctg cagccaacat gtccatagcc gtgaaggaac agcagaagaa 3061 ccagaagcct accaagtcgg tgtgggagca gcgcaccagc gagatgcgca agcagaatct 3121 gctggccagc cgcgaggcgc tgtacgggga cgcggctgag cgctggccca ccccctacgc 3181 gcgcccgctg cggcccgacg tgaagactca cttggaccgg ccgcttgtgg tggacccgca 3241 ggagaaccgt aacaacaaca ccaacaagag ccgtgcgcca gaagcgctgc gcccgaccgc 3301 gcggccccgc gagagcgcgc gcgaccccga cgcgcggcgc gcctggcccg gcagccctga 3361 gcgcgcccct ggacgagagg gcccgtatgg gcgcgagagc gagcctcagc agcgcgatca 3421 cgcgccgccc cgcgagcacg caccttggga cgcggacacg gagcgcgcca aagccgggga 3481 cgcgccccgc cgccacacgc accgacctgt ggcggagggc gagcctcgcc gccaccgcgc 3541 ccgccgccgg cccggggacg aacccgacga caggccagag cgcaggccgc gtccccgcga 3601 cgccactagg ccggcccgcg ctgcagacgg cgaaggcgac gatggggagc gcaagcggcg 3661 acaccgacac gggccgcccg cccacgacga cagggagcgc agacaccgac gcagaaaaga 3721 gaaccagggc tctggggtcc ccgtgtctgg tcccaacttg tccaccacca ggccaatcca 3781 gcaagacctg ggccgccagg acctgccgct ggctgaggat cttgacaaca tgaagaataa 3841 caagctggcc accggggaac ctgccagtcc ccacgacagc ctgggccaca gcggccttcc 3901 ccccagcccc gccaaaatcg ggaacagcac caaccctggt cccgccttgg ccaccaatcc 3961 ccagaacgct gccagccgca ggacgcccaa caatccgggc aacccgtcca accccggccc 4021 ccccaagact cccgagaaca gccttatcgt caccaacccc agcagcaccc agcccaactc 4081 agcgaagact gccaggaaac ccgagcacat ggcggtggag atccccccag cctgcccgcc 4141 cctcaaccac accgtggtcc aagtaaacaa aaacgccaac ccagacccac tgccaaagaa 4201 agaggaagag aagaaagagg aagaggaggc agaccccggg gaggacggcc caaagcccat 4261 gccgccctac agctccatgt tcatcctctc caccaccaac ccccttcgcc ggctgtgcca 4321 ttacatcctg aacctgcgct acttcgagat gtgcatcctc atggtcattg ccatgagcag 4381 catcgctctg gccgctgagg acccggtgca gcccaacgca ccccgcaaca atgtgctgcg 4441 atattttgac tatgttttta caggcgtgtt tacctttgag atggtgatca agatgatcga 4501 cctgggcctc gtcttgcatc agggggccta tttccgtgac ctatggaaca ttctggactt 4561 catagtggtc agtggggccc tggtggcctt tgccttcact ggcaatagca aaggaaagga 4621 cattaacacc attaagtctc tccgagtcct ccgggtgcta cgacctctaa agaccatcaa 4681 gcggctgcca aagctgaagg ccgtgtttga ctgcgtggtg aactcgctca agaacgtctt 4741 caacatcctc attgtgtaca tgctgttcat gttcatcttc gctgtggtag ccgtgcagct 4801 cttcaagggc aagttcttcc actgcacgga cgagtccaag gagttcgaga gagactgtcg 4861 aggcaagtac ctcctgtacg agaagaacga agtgaaggcc cgggaccgcg agtggaagaa 4921 gtacgagttc cactacgaca acgtgctctg ggctctgctc acacttttca cagtgtccac 4981 aggagagggc tggccacagg tcctcaagca ctcagtggat gctacttttg agaaccaggg 5041 ccccagcccc gggtaccgca tggagatgtc catcttctac gtggtgtact ttgtagtgtt 5101 tcccttcttc tttgtcaata tcttcgtggc cttgatcatc atcacctttc aggagcaggg 5161 agacaagatg atggaagagt acagcctgga gaaaaatgag agggcctgca tcgactttgc 5221 catcagtgcc aagccgctga ccaggcacat gccccagaac aagcagagct tccagtatcg 5281 aatgtggcag ttcgtcgtgt ccccaccctt tgagtacacc atcatggcca tgatcgctct 5341 caacaccatc gtgctaatga tgaagttcta cggagcctcg gtggcctatg aaaacgccct 5401 tcgcgtgttc aacattgtct tcacctccct cttctctctc gaatgtgtgc ttaaagtcat 5461 ggcttttggg attctgaatt atttccgcga tgcctggaac atcttcgact ttgtgactgt 5521 tctgggcagc atcacagaca tcctcgtcac cgagtttggg aataacttca tcaacctgag 5581 ctttctccgc ctcttccgtg ctgccagact catcaaactc ctccgccagg gttacaccat 5641 ccgcattctc ctctggactt tcgtgcagtc cttcaaggcc ctaccttatg tctgtctgct 5701 gatcgccatg ctcttcttca tctacgccat catcgggatg caggtgtttg gcaacatcgg 5761 cattgacggg gaagacgagg acagcgacga ggatgaattc caaatcacgg agcacaataa 5821 cttccggacc ttcttccaag cgctcatgct tctcttccgg agcgccacag gagaagcgtg 5881 gcacaacatc atgctctcct gcctcagcgg gaagccgtgc gataagaact ctgggatcct 5941 cacggcagac tgcggcaacg agttcgcgta cttctacttt gtctcgttca tcttcctgtg 6001 ctcgtttctg atgctgaatc tctttgtcgc tgtcatcatg gacaacttcg agtacctcac 6061 ccgagattct tccatcctgg gcccccatca cttggatgaa tacgtgcgtg tctgggcaga 6121 gtatgaccct gctgcctgcg gccgcattca ctataaggac atgtacagtt tattgcgagt 6181 aatatcgccc cctctcggct taggcaagaa atgtcctcat agggttgctt gcaagagact 6241 cctgcggatg gacctgccgg tagcagatga caacacagtt cacttcaact ccaccctgat 6301 ggctctgatc cgaaccgccc ttgatatcaa aattgccaag ggtggagctg acaagcagca 6361 gatggatgca gagcttcgca aggagatgat ggccatatgg cccaacctgt ctcagaagac 6421 cctggatctg ctggtcacac ctcacaagtc cacggacctg acagtgggta agatctacgc 6481 agccatgatg atcatggagt attaccggca gagcaaggcc aagaaactgc aggccatgcg 6541 agaggagcag aaccggacac cactcatgtt ccagcgcatg gagcctccat caccaacaca 6601 ggagggagga cccagccaga acgcccttcc ctccactcag ctggacccag gaggaggcct 6661 gatggctcac gaaggcggca tgaaggagag cccgtcctgg gtgacccagc gggcccaaga 6721 gatgttccag aagactggca cctggagccc agagcgaggg ccacccatcg acatgcctaa 6781 cagccagccc aactcccagt ctgtggagat gcgagaaatg ggtactgatg gctactcaga 6841 cagcgaacac tacctcccca tggaaggcca gaccagggcc gcctccatgc cccgcctccc 6901 agcagagaac cagaggagaa ggggccggcc acgtggaaat gacctcagta ccatctctga 6961 taccagcccc atgaagcgct cagcctctgt gctgggaccc aaagcccggc gactggatga 7021 ctactcacta gagcgggtac cgcctgagga gaaccaacgg taccaccaac gtcgccggga 7081 ccgtggccac cgcacctctg agcgctctct gggccgatac actgatgtgg acacaggttt 7141 ggggacagat ctgagcatga ccacccaatc gggtgacctg ccctccaaag atcgggacca 7201 ggaccggggc cgacccaagg accggaagca tcggccacac caccaccacc accaccacca 7261 tcatcatcat cccccggccc ccgaccggga tcgctacgca caggagcggc cggacaccgg 7321 ccgggcgcgg gcccgggagc agcgctggtc ccgctcgccc agcgaaggtc gggagcacac 7381 gacacacaga cagggcagta gttccgtcag tggaagccca gccccctcta cgtccggcac 7441 cagcacgccg cggcgcggcc gccgccagct cccccagacc ccctgcaccc cgcggccgct 7501 tgtgtcctac tcgcccgctc cgcgcaggcc tgcggcgcgc aggatggcgg gtcccgcagc 7561 gccccctggc ggctcgccgc ggggttgccg ccgtgcgccg cgctggcctg cgcatgcgcc 7621 tgagggcccg cggccgcgcg gtgccgacta caccgaaccc gacagcccgc gcgagccgcc 7681 agggggcgcg cacgatcccg cgccgcggtc gcccaggact ccgcgcgccg cgggctgcgc 7741 gtcaccgcgc cacggccgca ggctgcccaa tggttactac gcggggcacg gcgcgccgcg 7801 gccgcgcaca gcccgcaggg gcgcgcacga cgcgtacagc gagagcgagg acgactggtg 7861 ctaagccgcc ccgccccccg cagcgcccgt aaagtgcggg cgcactgcaa atgataaaca 7921 ataacgtttt taaaaaagaa aacccgggag aaaaacaaaa atagcttcta ttgatgagtt 7981 ttatcatctc gattgaatct ttcgttttcc cggtgaatga aaccgaggtg gctgcaagcc 8041 ggaaggacgg ctgcacgccc cacacacttc taagccgaat tatccagcac agtggcggcc 8101 gctcgagtct agagggccct tcgaaggtaa gcctatccct aaccctctcc tcggtctcga 8161 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc 8221 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 8281 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 8341 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 8401 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga 8461 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc 8521 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 8581 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 8641 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 8701 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 8761 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 8821 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 8881 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt 8941 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 9001 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 9061 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 9121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 9181 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt 9241 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct 9301 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca 9361 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa 9421 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag 9481 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac 9541 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt 9601 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac 9661 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc 9721 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac 9781 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc 9841 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 9901 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 9961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 10021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 10081 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 10141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 10201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 10261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 10321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 10381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 10441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 10501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 10561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 10621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 10681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 10741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 10801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 10861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 10921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 10981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 11041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 11101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 11161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 11221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 11281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 11341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 11401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 11461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 11521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 11581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 11641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 11701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 11761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 11821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 11881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 11941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 12001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 12061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 12121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 12181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 12241 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc 12301 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt 12361 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg 12421 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg 12481 ccagatatac gcgtt //
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